Studi Pathologis Cytopathological Effect Bovine Herpesvirus 1 Penyebab Infectious Bovine Rhinotracheitis dari Sapi pada Telur Ayam Berembrio

Yuli Purwandari Kristianingrum
Bagian Patologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
ORCID: https://orcid.org/0000-0002-0245-7632

Sitarina Widyarini
Bagian Patologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
ORCID: https://orcid.org/0000-0003-4461-8454

Tri Untari
Bagian Mikrobiologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Bambang Sutrisno
Bagian Patologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada

ABSTRACT
The objective of this study was to investigate about cytopathological effect (CPE) of Bovine Herpes Virus 1 that caused the Bovine Rhinotracheitis Infections (IBR) from bovine in the chorioallantoic membrane (CAM) of the embryonated chicken egg (ECE). Seven of swab nasal samples were taken from the bovine in the Breeding Farm of Padang Mengatas, West Sumatera. They were inoculated 0.5 mL in the chorioallantoic membrane of the 10 days ECE with thirth, repetitively. Before that, the samples were given antibiotic and antifungi treatment to protect from secondary infection. The observation was conducted first day until thirth day after infection. It was done to observe the pathological changes in the CAM of ECE eggs. The results showed that there was CPE as pock lesion in the CAM with variety size. The histopathologic changes appeared an acidophilic nuclear inclusion body in the epithelial of membrane. It showed that the virus could replicate in the nuclear of CAM. The conclusion of this study was IBR virus has CPE in CAM of ECE with a specific pock lesion.

Keywords: CAM, IBR, ECE, pock lesion